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pcmv vsv g envelope vectors  (Addgene inc)


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    Addgene inc pcmv vsv g envelope vectors
    Pcmv Vsv G Envelope Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enveloping+vector+pcmv+vsv+g/pCMV-VSV-G+(Plasmid+%238454)/pmc12701018-32-14-17
    Average 96 stars, based on 3094 article reviews
    pcmv vsv g envelope vectors - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transduction:

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packaging vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells.
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packag ing vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.

    Generated:

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packaging vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells.
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packag ing vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.

    shRNA:

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packaging vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells.
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packag ing vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.

    Plasmid Preparation:

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packaging vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.

    Article Title: Host cell sensing and restoration of mitochondrial function and metabolism within Helicobacter pylori VacA intoxicated cells.
    Article Snippet: .. Lentiviral particles used for transduction were generated in HEK293T cells that were co-transfected with 0.5 μg/mL shRNA-carrying plasmid, 0.45 μg/mL packag ing vector pCMV-dR8.2 (plasmid# 8455, Addgene, Watertown, MA), and 0.05 μg/mL enveloping vector pCMV-VSV-G (plasmid# 8454, Addgene) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). .. Lentiviral clones expressing shRNA against human PRKAA1 (AMPKα1) (TRCN0000196482, Sigma-Aldrich), human PRKAA2 (AMPKα2) (TRCN0000002171, Sigma-Aldrich), and human Drp-1 shRNA (sc-43732-SH, Santa Cruz Biotechnology) were used for generating lentiviral particles.



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